Introduction: The surface of the cortex is formed by bodies of neural cells that process all the information that the brain receives and sends, enabling man to think, plan, coordinate thoughts and actions, perceive visual and sound patterns and use languages. The prefrontal cortex (CPF) is a complex and highly developed region of the neocortical regions of the human brain, being considered the frontal cortex association cortex. Objective: To analyze the effects of supplementation with different dosages of Phosphatidylserine on the neuroprotection and plasticity of nerve cells in the prefrontal cortex of cultured newborn Wister rats. Methods: the experimental procedures were conducted under license from the Ethics Committee on Animal Experimentation (CEEA) of the State University of Rio Grande do Norte (UERN), with opinion number 015/2016. The cellular material of the cerebral cortex was collected from 2 newborn Wistar rats, 2 days old. Then cultivated in different groups, one control (D10 = G1) and the others, containing different concentrations of phosphatidylserine, PS (G2 = 1 μg, G3 = 2 μg and G4 = 3 μg). Scanning electron microscopy (SEM) was used to evaluate growth and cell morphology after 72 hours. To verify normality, the Kolmogorov-Smirnov and Shapiro-Wilk tests were used and to confirm the results, two post-tests were performed – Tukey and Bonferroni – with the difference results with p <0.05 being considered statistically significant. Results: We evidenced that the group G4 treated with 3 μg of the phosphatidylserine conditioned medium, presented significant morphological growth when compared to the groups G1 = D10, G2 = 1 μg and G3 = 2 μg, suggesting that this phosphate may act promoting neuroprotection and plasticity to neurons of the cerebral cortex, in culture. Conclusion: The neuroprotective effect and the preservation of neuroplasticity of cells in culture are notorious. By the way, phosphatidylserine promoted the maintenance of cell expansion in the experimental groups, mainly in the group treated with conditioned medium of 3μg, allowing the more expressive identification of the cell morphology, indicating its action in the neuroprotection of the cerebral cortex cells.
Keywords: Pre-frontal córtex. Phosphatidylserine. Neuroprotection. Cerebral Plasticity.
Rosângela Symara Lima Araújo;
Amália Cinthia Meneses do Rêgo;
Fausto Pierdoná Guzen.