Trophic Performance of Neurons of the Prefrontal Cortex of Newborn Wistar Rats in Culture After Addition of Phosphatidylserine

Abstract:
Introduction: The surface of the cortex is formed by bodies of neural cells that process all the infor­mation that the brain receives and sends, enabling man to think, plan, coordinate thoughts and ac­tions, perceive visual and sound patterns and use languages. The prefrontal cortex (CPF) is a com­plex and highly developed region of the neocorti­cal regions of the human brain, being considered the frontal cortex association cortex. Objective: To analyze the effects of supplementation with different dosages of Phosphatidylserine on the neuroprotection and plasticity of nerve cells in the prefrontal cortex of cultured newborn Wister rats. Methods: the experimental procedures were con­ducted under license from the Ethics Committee on Animal Experimentation (CEEA) of the State University of Rio Grande do Norte (UERN), with opinion number 015/2016. The cellular material of the cerebral cortex was collected from 2 newborn Wistar rats, 2 days old. Then cultivated in diffe­rent groups, one control (D10 = G1) and the others, containing different concentrations of phosphatidylserine, PS (G2 = 1 μg, G3 = 2 μg and G4 = 3 μg). Scanning electron microscopy (SEM) was used to evalua­te growth and cell morphology after 72 hours. To verify normality, the Kolmo­gorov-Smirnov and Shapiro-Wilk tests were used and to confirm the results, two post-tests were performed – Tukey and Bonferroni – with the difference results with p <0.05 being considered statisti­cally significant. Results: We evidenced that the group G4 treated with 3 μg of the phosphatidylserine conditioned me­dium, presented significant morphologi­cal growth when compared to the groups G1 = D10, G2 = 1 μg and G3 = 2 μg, su­ggesting that this phosphate may act pro­moting neuroprotection and plasticity to neurons of the cerebral cortex, in culture. Conclusion: The neuroprotective effect and the preservation of neuroplasticity of cells in culture are notorious. By the way, phosphatidylserine promoted the maintenance of cell expansion in the ex­perimental groups, mainly in the group treated with conditioned medium of 3μg, allowing the more expressive identifica­tion of the cell morphology, indicating its action in the neuroprotection of the cerebral cortex cells.

Keywords:  Pre-frontal córtex. Phosphatidylserine. Neuroprotection. Cerebral Plasticity.

Authors:
Rosângela Symara Lima Araújo;
Amália Cinthia Meneses do Rêgo;
Fausto Pierdoná Guzen.