Sciatic nerve-conditioned medium promotes trophic induction in spinal cord cultured cells

Abstract:

The fibers in the peripheral nervous system are frequent targets of injuries, being distributed throughout the length of the body. Such lesions culminate in motor, sensory and autonomic changes, thus recent evidence shows the influence ideal environment for growth of nerve fibers damaged in the Central Nervous System (CNS). Our objective was to verify this cell behavior in the central sphere, analyzing cellular plasticity of the spinal cord (SC) in the presence of sciatic nerve-conditioned medium (SNCM) from neonatal rats. 12 animals were used, 06 of those aged 45 days, and the other 06 aged 02 days, and it was approved by the Ethics Committee on Animal Experimentation nº007/13. It was inoculated SNCM from explants of the sciatic nerve of the animals in the SC neurons culture aiming the trophic behavior of these cells by morphometric analysis and immunocytochemistry for microtubule-associated protein-2 at the end of 72 hours. The neurons and glial cells of the SC showed a typical morphology and rapid expansion, particularly in samples that higher dosage of SNCM were exposed when compared to the group devoid of SNCM (p≤0,0001). However, this study improves the understanding of the plasticity of neurons and glial cells in the SC and facilitates understanding in the search for better techniques with vehicle management and cell therapy in the use of disease and trauma in the CNS. The trophic role of the SNCM made it possible to register the patent in the organ of the National Institute of Intellectual Property (n° BR 1020160162289).


Keywords: Spinal Cord. Conditioned medium. Sciatic nerve. Plasticity

Authors:
Renato Serquiz e Pinheiro;
Stephan Barisic Júnior;
Flávio Henrique Amaral Pires Véras;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Eudes Euler de Souza Lucena;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho;
Dinalva Brito de Queiroz and Fausto Pierdoná Guzen

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