Sciatic nerve-condicioned medium with the addition of methylprednisolone promotes morphological plasticity in cultured spinal cord neurons

Abstract:

Introduction: Trauma is an important cause of morbidity and mortality worldwide, constituting a growing problem that is affecting young people who are in good health and are economically active. Spinal Cord Injury (SCI) is often a result of trauma, and it can be minimized by the actions of neuroprotective drugs. Evidence shows that methylprednisolone (MP) is a strong anti-inflammatory component, fighting lipid oxidation and thereby reducing the degeneration of the nervous system. Drugs associated with sciatic nerve-conditioned medium (SNCM), which provides an environment that is rich in substances that influence the growth of injured nerve fibres in the peripheral nervous system (PNS), have been studied. Objective: Within this neuropharmaceutical perspective, this study analysed the cellular plasticity of spinal cord neurons in the presence of SNCM rats before the addition of MP. Method: Cell morphology was assessed over 72 hours, and immunocytochemistry analyses were conducted for β-tubuline III and NF-200 Result: Morphological analysis showed evident trophic development in the group that received the SNCM in the presence of MP (p≤0.0001), and immunoreactivity for β-tubulina III and NF-200 was more evident. Conclusion: This study of the plasticity of neurons of the spinal cord opened prospective new techniques for cell therapy in the presence of SNCM and MP, which promotes neuroprotective action.


Keywords: Cell Culture. Methylprednisolone. Neuronal Plasticity. Sciatic Nerve

Authors:
Salvador Viana Gomes Júnior;
Cleber Mahlmann Viana Bezerra;
Eudes Euler de Souza Lucena;
José Rodolfo Lopes de Paiva Cavalcanti;
Dayane Pessoa de Araújo;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho and Fausto Pierdoná Guzen

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