Conditioned medium of sympathetic ganglia with addition of fibroblast growth factor 2 promotes plasticity of cardiomyocytes in culture

Abstract:

Introduction: The cardiac muscle cell, also called the cardiomyocyte, has specialized functions, playing a fundamental role in maintaining life, since it is responsible for the electrical and contractile activity of the heart. Like other specialized cells, it does not have an effective capacity for regeneration and its death or apoptosis is always an undesirable event, with sometimes catastrophic consequences, such as in Heart Failure (HF), which is known as the “final path” of heart diseases. Howe Conditioned ver, any cardiac disease in which cardiomyocyte apoptosis is implicated will develop, at some point in time, the HF syndrome. Evidence shows the influence of several substances as strong components responsible for inducing cardioprotection after tissue damage. Objective: In this perspective, this study aimed to analyze the plasticity of cardiomyocytes in the presence or absence of Fibroblast Growth Factor 2 (FGF-2) in the presence of sympathetic ganglia conditioned medium (MCGS). Method: For this, the behavior of the sympathetic ganglion in culture was analyzed, morphologically mapping the population and the ganglionar migratory profile, the growth and the morphology of the cardiomyocytes over 72 hours through the phase contrast microscopy, protocol number 006/15. Results: A statistically significant increase was observed both in the area and the cell perimeter in the groups treated with MCGS and MCGS with addition of FGF-2 (p = 0.0001) in relation to the control group. Conclusion: It is concluded, therefore, that there is an important plastic effect of MCGS, potentiated by FGF-2 in cultured cardiomyocytes.

Keywords: Cardiomyocyte. Fibroblast Growth Factor 2. Sympathetic Ganglion. Conditioned Medium

Authors:
Stephan Barisic Júnior;
Flávio Henrique Amaral Pires Véras;
Ianara Mendonça da Costa;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
José Rodolfo Lopes de Paiva Cavalcanti;
Eudes Euler de Souza Lucena;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho;
Dinalva Brito de Queiroz, and Fausto Pierdoná Guzen

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