Author page: JPCBS

Supplementation’s effects of polyunsaturated fatty acids omega 3 in Alzheimer’s disease animal models: a systematic review

Abstract:

Introduction: There are few randomized clinical trials that studied the effects of omega-3 polyunsaturated fatty acids (ω3 PUFA) on Alzheimer’s Disease (AD). Some of these studies showed that patients with mild cognitive impairment have benefits in the treatment, but none of them showed significant improvement in cognitive function in patients with advanced or moderate AD. All these randomized clinical trials had relatively short duration of supplementation, therefore, one of the reasons that may not have contributed to improvement in patients with moderate and advanced disease would be the short period of study. Animal studies offer better long-term controlled research possibilities, compared to clinical studies. Methods: Therefore, a systematic literature review was conducted that focused on the effects of the relevance of long-term ω3 PUFA supplementation on cognitive impairment and neuronal loss in AD animal models. Results: This systematic review showed that the long term, the ω3 PUFA supplementation decreased the ratio omega-6 / omega-3 reduced neuronal loss in experimental models of AD, as well as improved cognitive function. This effect was more evident in older mice compared to young mice, and compared with males females. Conclusions: These results indicate the importance of new clinical trials be conducted with long-term ω3 PUFA supplementation in patients with AD for possible associations correct dosages in the treatment.

Keywords: Omega-3. Alzheimer’s Disease. Cognition

Authors:
Euglena Lessa Bezerra;
Branteli Martins Machado;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Ianara Mendonça da Costa;
Dinalva Brito de Queiroz;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho and Fausto Pierdoná Guzen

Epileptic Seizures: the challenge continues

Abstract:

Introduction: Epilepsy is one of the most prevalent primary central nervous system diseases in the world. This pathology is classified into two principal axes: topographic and etiological. In most cases, the diagnosis of an epileptic seizure can be made clinically by obtaining a detailed history and general physical examination, with neurological and psychiatric emphasis. Methods: This is the literature review study, whose bibliographic research was carried out in Pubmed, Scopus, Scielo and Web of Science. The descriptors used epilepsy, seizure disorder, tonic-clonic seizure syndrome; status epilepticus, grand mal status epilepticus and treatment outcome. The inclusion criteria, were selected articles published in English, Spanish and Portuguese; Randomized Clinical Trials / Clinical Trial), Guidelines (Guideline), Systematic Reviews, Reviews and Meta-Analysis. Results: The investigation and follow-up of the first convulsive crisis differ from that in patients with epilepsy. If necessary, the choices of antiepileptics should be made according to the type of crisis, comorbidities, and socioeconomic conditions. Conclusions: This study aimed to review and discuss, in a practical way, a therapeutic approach to epileptic seizures.

Keywords:
Epilepsy. Seizure disorder. Tonic-clonic seizure syndrome. Status epilepticus. Grand mal status epilepticus. Treatment outcome

Authors:
Renato Serquiz e Pinheiro, Yanny Cinara Ernesto;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho and Fausto Pierdoná Guzen

Addition of FGF-2 to sciatic nerve-conditioned medium to promote plasticity in spinal cord cultured cells

Abstract:

Introduction: Spinal cord injuries (SCI) usually result in crippling conditions due to the very limited repair ability of the mammalians’ central nervous system (CNS). A favorable environment for cell plasticity after a spinal cord manipulation can be created through a combination of peripheral nerve graft transplantation and neurotrophic factors. Objective: This study aims to investigate the role of FGF-2 in the cellular plasticity of the spinal cord in the presence of sciatic nerve-conditioned medium (SNCM) in rats. Methods: Cell morphology was assessed over 72 hours, and immunocytochemistry analyses were conducted for NF200 and GFAP. Results: Morphological analysis showed evident trophic development in the group that received SNCM in the presence of FGF-2 (p≤0.0001). Moreover, immunoreactivity for NF200 and GFAP was more evident and plastic compared to that of the group without FGF-2. Conclusions: The evident increase in the plasticity of spinal cord cells might open new perspectives for the development of techniques for cell therapy using the association of SNCM with FGF-2.

Keywords: Conditioned medium. Fibroblast growth factor-2. Neuroplasticity. Sciatic nerve. Spinal cord

Authors:
Cleber Mahlmann Viana Bezerra;
Eudes Euler de Souza Lucena;
Eduardo Pereira de Azevedo, Dinalva Brito de Queiroz;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho;
Francisco Irochima Pinheiro;
Miriam Stela Maris de Oliveira Costa and Fausto Pierdoná Guzen

Culture Technique of Human Nervous Cells Collected from Traumatized Brains

Abstract:

Introduction: The human brain is composed of a complex network of cells that interact with each other through direct and indirect pathways. Studies about culture of human brain cells have been limited to postmortem experiments and only more recently have been obtained from living patients. These harvests of human nerve tissue samples occurred only during neurosurgeries for control of refractory epilepsy and resection of brain tumors. Methods: We describe in this work a technique for culture of nerve cells removed during urgency neurosurgeries in injured brains (patients who suffered traumatic brain injury and needed surgery), aiming the culture of injured cells for later study of nerve regeneration. These samples of nerve tissue were collected from carefully selected patients: in cases of depressed skull fracture with laceration of the dura-mater and cerebral cortex, with spontaneous exit (caused by trauma) of brain tissue, which obviously required neurosurgery; and in patients who presented traumatic intracranial hematomas with subcortical component and expansive effect, requiring surgical removal through a small opening in the cerebral cortex, known as corticectomy, for drainage of the clot. After obtaining, this material was immediately stored and sent to treatment and plating, being maintained in culture medium in a CO2 incubator. Results: In the medium, the presence of neurons and glial cells can be verified few days later. Conclusions: It is concluded that is possible to culture injured nerve cells through the technique employed, thus opening a field of research for the study of substances with presumed neuroprotective function.


Keywords: Cell culture techniques. Craniocerebral trauma. Neuroglia. Neurons

Authors:
Starlynn Freire dos Santos;
Ianara Mendonça da Costa;
Francisca Idalina Neta;
Eudes Euler Sousa Lucena;
José Rodolfo Lopes de Paiva Cavalcanti;
Dinalva Brito de Queiroz;
Amália Cinthia Meneses do Rêgo;
Francisco Irochima Pinheiro;
Irami Araújo-Filho and Fausto Pierdoná Guzen

Prevalence and risk factors associated with perinatal asphyxia in newborn infants

Abstract:

Introduction: Perinatal asphyxia is defined as an injury to the fetus or to the newborn caused by lack of oxygen (hypoxia) and/ or lack of perfusion to some organs (ischemia), which is enough to induce biochemical and functional consequences. Objective: To determine the prevalence and main risk factors associated with perinatal asphyxia in a group of neonates. Methods: This is a cross-sectional, quantitative and descriptive exploratory study, conducted through interviews with puerperal women who gave birth in hospitals located in a city of the Rio Grande do Norte state, Brazil. 1079 individuals participated of the study where 18 cases of asphyxiated newborns were identified. The inclusion criteria were neonates born with at least 22 weeks of gestational age with Apgar score lower than 3 in the first minute or less than 7 in the fifth minute (study group) compared to the control group (Apgar equal to or greater than 8 and 10 in the first and fifth minutes, respectively). Results: Multivariate analysis revealed an association between perinatal asphyxia with the number of prenatal consultations (1.293-1.779, P = 0.030), uterine bleeding (0.021-0.934, P = 0.042) and sedation of the mother (0.009-0.203, P = 0.001). After logistic analysis, the variables that remained in the model were anemia (1.820-40.874, P = 0.031), high risk of preterm birth (2.32331.529, P = 0.009), pregnancy bleeding (1.934-25.691, P = 0.015) and hospitalization during pregnancy (1.174-8.247, P = 0.016). Conclusion: Information about the birth profile can direct the actions of perinatal care and the conduction of preventive practices toward pregnant women with the purpose of reducing the likelihood of perinatal asphyxia.


Keywords: Perinatal asphyxia. Prevalence. APGAR scoring Newborn

Authors:
Rejane Maria de Oliveira Holanda;
Ana Maria de Lima Dantas;
Ianara Mendonça da Costa;
Eudes Euler de Souza Lucena;
Lucídio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Eduardo Pereira de Azevedo;
Francisco Irochima Pinheiro;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho and Fausto Pierdoná Guzen

Perception of body image among candidates for aesthetic plastic surgery

Abstract:

Introduction: The aim of this study is to investigate the body image perception among women seeking aesthetic plastic surgery. The personality and body image of women seeking esthetic plastic surgery were investigated through the evaluation. Methods: The data were collected using their personal medical records and through the application of a questionnaire, the Body Shape Questionnaire (BSQ), which evaluates the level of concern about the body. In addition, the Silhouette Scale was used, which evaluates the perception that the woman has of her own body. The variables of body mass index and silhouettes as a function of the BSQ scale were compared by analysis of variance ANOVA. Results: The results showed that the women used in this study had distortions of their body image perception and that the degree of concern with their body and BMI interfered in such distorted perception (p=0.0000), which evidenced the dissatisfaction of the women with their self-image. Conclusions: This study demonstrated that distortions of women’s perception of real body image occur and that the degree of concern with their body and BMI interferes with this perception. The increasing number of women seeking for plastic surgery evidences the dissatisfaction with their self-image.

Keywords: Body image. Plastic surgery. Self-esteem

Authors:
Ana Maria de Lima Dantas;
Rejane Maria de Oliveira Holanda;
Ianara Mendonça da Costa;
Eudes Euler de Souza Lucena;
Lucídio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Eligleidson José Vidal de Oliveira;
Eduardo Pereira de Azevedo;
Francisco Irochima Pinheiro;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho and Fausto Pierdoná Guzen

Conditioned medium of sympathetic ganglia with addition of fibroblast growth factor 2 promotes plasticity of cardiomyocytes in culture

Abstract:

Introduction: The cardiac muscle cell, also called the cardiomyocyte, has specialized functions, playing a fundamental role in maintaining life, since it is responsible for the electrical and contractile activity of the heart. Like other specialized cells, it does not have an effective capacity for regeneration and its death or apoptosis is always an undesirable event, with sometimes catastrophic consequences, such as in Heart Failure (HF), which is known as the “final path” of heart diseases. Howe Conditioned ver, any cardiac disease in which cardiomyocyte apoptosis is implicated will develop, at some point in time, the HF syndrome. Evidence shows the influence of several substances as strong components responsible for inducing cardioprotection after tissue damage. Objective: In this perspective, this study aimed to analyze the plasticity of cardiomyocytes in the presence or absence of Fibroblast Growth Factor 2 (FGF-2) in the presence of sympathetic ganglia conditioned medium (MCGS). Method: For this, the behavior of the sympathetic ganglion in culture was analyzed, morphologically mapping the population and the ganglionar migratory profile, the growth and the morphology of the cardiomyocytes over 72 hours through the phase contrast microscopy, protocol number 006/15. Results: A statistically significant increase was observed both in the area and the cell perimeter in the groups treated with MCGS and MCGS with addition of FGF-2 (p = 0.0001) in relation to the control group. Conclusion: It is concluded, therefore, that there is an important plastic effect of MCGS, potentiated by FGF-2 in cultured cardiomyocytes.

Keywords: Cardiomyocyte. Fibroblast Growth Factor 2. Sympathetic Ganglion. Conditioned Medium

Authors:
Stephan Barisic Júnior;
Flávio Henrique Amaral Pires Véras;
Ianara Mendonça da Costa;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
José Rodolfo Lopes de Paiva Cavalcanti;
Eudes Euler de Souza Lucena;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho;
Dinalva Brito de Queiroz, and Fausto Pierdoná Guzen

Spontaneous Subarachnoid Hemorrhage: updated clinical and therapeutic approach

Abstract:

Despite advances in the treatment of subarachnoid hemorrhage, morbimortality rates remain elevated. Patients who have a sudden onset headache followed or not by altered consciousness, require a high degree of suspicion for the appropriate diagnosis in the emergency room. Those with lighter symptoms, presence of headache without other neurological alterations are the most susceptible to diagnostic error. All should be evaluated quickly, receiving specialized neurointensive care and ear
Spontaneous ly treatment. The best results are usually obtained from the individualized discussion of each case, given the clash between surgical and endovascular treatment. Finally, it is safe to prevent, recognize and treat systemic delayed cerebral ischemia and vasospasm. The present study aimed to review and discuss, in a practical way, the approach of spontaneous subarachnoid hemorrhages.

Keywords: Subarachnoid hemorrhage. Intracranial subarachnoid hemorrhages. Intracranial aneurysm. Physiopathology. Clinical decision rules. Neurologic surgical procedure. Interventional radiology

Authors:
Renato Serquiz e Pinheiro;
Yanny Cinara T. Ernesto;
Amália Cinthia Meneses Rêgo;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Dinalva Brito de Queiroz;
Fausto Pierdoná Guzen and Irami Araújo-Filho

Sciatic nerve-conditioned medium promotes trophic induction in spinal cord cultured cells

Abstract:

The fibers in the peripheral nervous system are frequent targets of injuries, being distributed throughout the length of the body. Such lesions culminate in motor, sensory and autonomic changes, thus recent evidence shows the influence ideal environment for growth of nerve fibers damaged in the Central Nervous System (CNS). Our objective was to verify this cell behavior in the central sphere, analyzing cellular plasticity of the spinal cord (SC) in the presence of sciatic nerve-conditioned medium (SNCM) from neonatal rats. 12 animals were used, 06 of those aged 45 days, and the other 06 aged 02 days, and it was approved by the Ethics Committee on Animal Experimentation nº007/13. It was inoculated SNCM from explants of the sciatic nerve of the animals in the SC neurons culture aiming the trophic behavior of these cells by morphometric analysis and immunocytochemistry for microtubule-associated protein-2 at the end of 72 hours. The neurons and glial cells of the SC showed a typical morphology and rapid expansion, particularly in samples that higher dosage of SNCM were exposed when compared to the group devoid of SNCM (p≤0,0001). However, this study improves the understanding of the plasticity of neurons and glial cells in the SC and facilitates understanding in the search for better techniques with vehicle management and cell therapy in the use of disease and trauma in the CNS. The trophic role of the SNCM made it possible to register the patent in the organ of the National Institute of Intellectual Property (n° BR 1020160162289).


Keywords: Spinal Cord. Conditioned medium. Sciatic nerve. Plasticity

Authors:
Renato Serquiz e Pinheiro;
Stephan Barisic Júnior;
Flávio Henrique Amaral Pires Véras;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Eudes Euler de Souza Lucena;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho;
Dinalva Brito de Queiroz and Fausto Pierdoná Guzen

Sciatic nerve-condicioned medium with the addition of methylprednisolone promotes morphological plasticity in cultured spinal cord neurons

Abstract:

Introduction: Trauma is an important cause of morbidity and mortality worldwide, constituting a growing problem that is affecting young people who are in good health and are economically active. Spinal Cord Injury (SCI) is often a result of trauma, and it can be minimized by the actions of neuroprotective drugs. Evidence shows that methylprednisolone (MP) is a strong anti-inflammatory component, fighting lipid oxidation and thereby reducing the degeneration of the nervous system. Drugs associated with sciatic nerve-conditioned medium (SNCM), which provides an environment that is rich in substances that influence the growth of injured nerve fibres in the peripheral nervous system (PNS), have been studied. Objective: Within this neuropharmaceutical perspective, this study analysed the cellular plasticity of spinal cord neurons in the presence of SNCM rats before the addition of MP. Method: Cell morphology was assessed over 72 hours, and immunocytochemistry analyses were conducted for β-tubuline III and NF-200 Result: Morphological analysis showed evident trophic development in the group that received the SNCM in the presence of MP (p≤0.0001), and immunoreactivity for β-tubulina III and NF-200 was more evident. Conclusion: This study of the plasticity of neurons of the spinal cord opened prospective new techniques for cell therapy in the presence of SNCM and MP, which promotes neuroprotective action.


Keywords: Cell Culture. Methylprednisolone. Neuronal Plasticity. Sciatic Nerve

Authors:
Salvador Viana Gomes Júnior;
Cleber Mahlmann Viana Bezerra;
Eudes Euler de Souza Lucena;
José Rodolfo Lopes de Paiva Cavalcanti;
Dayane Pessoa de Araújo;
Lucidio Clebeson de Oliveira;
Luciana Cristina Borges Fernandes;
Amália Cinthia Meneses do Rêgo;
Irami Araújo-Filho and Fausto Pierdoná Guzen

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